Sladek Research Group

Bin Fang, Daniel Mane-Padros, Eugene Bolotin, Tao Jiang, Frances M. Sladek Identification of a binding motif specific to HNF4 by comparative analysis of multiple nuclear receptors Nucleic Acids Research. Epub 2012 Mar 01. PMID: 22383578 [PubMed - indexed for MEDLINE]

Karthikeyani Chellappa, Lucy Jankova, Jake M. Schnabl, Songqin Pan, Yann Brelivet, Caroline L-S. Fung, Charles Chan, Owen F. Dent, Stephen J. Clarke, Graham R. Robertson, and Frances M. Sladek Src tyrosine kinase phosphorylation of nuclear receptor HNF4a correlates with isoform-specific loss of HNF4a in human colon cancer PNAS. Epub 2012 Jan 30. PMID: 22308320 [PubMed - indexed for MEDLINE]

Eugene Bolotin, Karthikeyani Chellappa, Wendy Hwang-Verslues, Jake M Schnabl, Chuhu Yang and Frances M SladekNuclear Receptor HNF4a Binding Sequences are Widespread in Alu Repeats BMC Genomics. Epub 2011 Nov 15. PMID: 22085832 [PubMed - indexed for MEDLINE]

Hwang-Verslues WW, Sladek FM. HNF4a-role in drug metabolism and potential drug target? Curr Opin Pharmacol. Epub 2010 Sep 09. PMID: 20833107 [PubMed - indexed for MEDLINE]

Bolotin E, Schnabl J, Sladek FM  HNF4A (Homo sapiens).Transcription Factor Encyclopedia. 2010

Sladek FM. What are nuclear receptor ligands? Molecular and Cellular Endocrinology. Epub 2010 June 06. PMID: 20615454 [PubMed - indexed for MEDLINE] [PDF]

Bolotin E, Liao H, Ta TC, Hwang-Verslues W, Evans J, Jiang T, and Sladek FM. Integrated Approach for the Identification of Human HNF4? Target Genes Using Protein Binding Microarrays. Hepatology. 2010 Feb;51(2):642-53. PMID: 20054869 [PubMed - indexed for MEDLINE] 

Yuan X*, Ta J*, Lin M, Evans J, Dong Y, Bolotin E, Sherman MA, Forman BM*, Sladek FM* . Identification of an endogenous ligand bound to a native orphan nuclear receptor. PLoS ONE. 2009;4(5):e5609. Epub 2009 May 09. PMID: 19440305 [PubMed - indexed for MEDLINE] (*contributed equally)

Xie X, Liao H, Dang H, Pang W, Guan Y, Wang X, Shyy JY, Zhu Y*, Sladek FM*. Down regulation of hepatic HNF4a gene expression during hyperinsulinemia via SREBPs. Mol Endocrinol. 2009 Apr;23(4):434-43. Epub 2009 Jan 29. PMID: 19179483 [PubMed - indexed for MEDLINE] (*contributed equally)

Nammo T, Yamagata K, Tanaka T, Kodama T, Sladek FM, Fukui K, Katsube F, Sato Y, Miyagawa J, Shimomura I. Expression of HNF-4alpha (MODY1), HNF-1beta (MODY5), and HNF-1alpha (MODY3) proteins in the developing mouse pancreas. Gene Expr Patterns. 2008 Jan;8(2):96-106. Epub 2007 Oct 9. PMID: 17996499 [PubMed - indexed for MEDLINE]

Hwang-Verslues WW, Sladek FM.  Nuclear receptor hepatocyte nuclear factor 4alpha1 competes with oncoprotein c-Myc for control of the p21/WAF1 promoter. Mol Endocrinol. 2008 Jan;22(1):78-90. Epub 2007 Sep 20. PMID: 17885207 [PubMed - indexed for MEDLINE]

Sun K, Montana V, Chellappa K, Brelivet Y, Moras D, Maeda Y, Parpura V, Paschal BM, Sladek FM.  Phosphorylation of a conserved serine in the deoxyribonucleic acid binding domain of nuclear receptors alters intracellular localization.Mol Endocrinol. 2007 Jun;21(6):1297-311. Epub 2007 Mar 27. PMID: 17389749 [PubMed - indexed for MEDLINE]

Yang C, Bolotin E, Jiang T, Sladek FM, Martinez E. Prevalence of the initiator over the TATA box in human and yeast genes and identification of DNA motifs enriched in human TATA-less core promoters. Gene. 2007 Mar 1; 389(1):52-65. Epub 2006 Oct 10. PMID: 17123746 [PubMed - indexed for MEDLINE]

Benoit, G., A. Cooney, V. Giguere, H. Ingraham, M. Lazar, G Muscat, T. Perlmann, JP Renaud, J Schwabe, FM Sladek, MJ Tsai and V. Laudet (2006) International Union of Pharmacology. LXVI. Orphan Nuclear Receptors. Pharmacological Reviews 58: 798-836.

Maeda Y, Hwang-Verslues WW, Wei G, Fukazawa T, Durbin ML, Owen LB, Liu X, Sladek FM. Tumour suppressor p53 down-regulates the expression of the human hepatocyte nuclear factor 4alpha (HNF4alpha) gene. Biochem J. 2006 Dec 1;400(2):303-13. PMID: 16895524 [PubMed - in process]

Battle MA, Konopka G, Parviz F, Gaggl AL, Yang C, Sladek FM, Duncan SA. Hepatocyte nuclear factor 4{alpha} orchestrates expression of cell adhesion proteins during the epithelial transformation of the developing liver. Proc Natl Acad Sci USA. 2006 May 30;103(22):8419-24. Epub 2006 May 19.PMID: 16714383 [PubMed - in process]

Garrison WD, Battle MA, Yang C, Kaestner KH, Sladek FM, Duncan SA. Hepatocyte nuclear factor 4alpha is essential for embryonic development of the mouse colon.Gastroenterology. 2006 Apr;130(4):1207-20. PMID: 16618389 [PubMed - indexed for MEDLINE]

Zhang Y, Lee TS, Kolb EM, Sun K, Lu X, Sladek FM, Kassab GS, Garland T Jr,Shyy JY.  AMP-activated protein kinase is involved in endothelial NO synthase activation in response to shear stress. Arterioscler Thromb Vasc Biol. 2006 Jun;26(6):1281-7. Epub 2006 Apr 6.PMID: 16601232 [PubMed - in process]

Miura A, Yamagata K, Kakei M, Hatakeyama H, Takahashi N, Fukui K, Nammo T,Yoneda K, Inoue Y, Sladek FM, Magnuson MA, Kasai H, Miyagawa J, Gonzalez FJ, Shimomura I. Hepatocyte nuclear factor-4alpha is essential for glucose-stimulated insulinsecretion by pancreatic beta-cells. J Biol Chem. 2006 Feb 24;281(8):5246-57. Epub 2005 Dec 23.PMID: 16377800 [PubMed - indexed for MEDLINE]

Ihara A, Yamagata K, Nammo T, Miura A, Yuan M, Tanaka T, Sladek FM, Matsuzawa Y, Miyagawa J, Shimomura I. Functional characterization of the HNF4alpha isoform (HNF4alpha8) expressed in pancreatic beta-cells. Biochem Biophys Res Commun. 2005 Apr 15;329(3):984-90.PMID: 15752752 [PubMed - indexed for MEDLINE]

T.Fukazawa*, Y.Maeda*, F.M. Sladek, and L.B. Owen-Schaub (2004). Development of a Cancer Targeted Tissue Specific Promoter System. Cancer Research, 64: 363-369. *Contributed equally.

F.M. Sladek (2003). Nuclear Receptors as Drug Targets: new Developments in Coregulators, orphan receptors and Major Therapeutic Areas. Expert Opinion Therapeutic Targets 7: 1-6 [PDF]

Q-J Li, S-H Yang, Y. Maeda, F.M. Sladek, A.D. Sharrocks, and M. Martins-Green (2003). MAP kinase phosphroylation-dependent activation of ELK-1leads to activation of the coactivator p300 EMBO, Vol. 22, No. 2 pp. 281-291.

F.M. Sladek (2002). Desperately seeking... something.Molecular Cell 10:(2) 219-221.

M. Torres-Padilla, F.M. Sladek and M. Weiss (2002). Developmentally regulated N-terminal variants of the nuclear receptor HNF4a mediate multiple interactions through coactivator and corepresor/HDAC complexes. Journal of Biological Chemistry, 277: 44677-44687

K. Ellrott, C.Yang, F.M. Sladek and T. Jiang (2002). Identifying transcription factor binding sites through Markov chain optimization. Bioinformatics, 18, Suppl. 2, S1-S10.

Y. Maeda, C. Rachez, L. Hawel, III, C.V. Byus, L.P. Freedman and F.M. Sladek (2002).  Polyamines modulate the interaction between nuclear receptors and DRIP205. Molecular Endocrinology 16:1502-10.

M.D. Ruse,Jr,M. Privalsky and F.M. Sladek (2002). Competitive Cofactor Recruitment by Orphan receptor HNF4a: Modulation by the F domain. Molecular and Cellular Biology 22:1626-1638.

Y. Maeda, S. D. Seidel, G. Wei, X. Liu and F.M. Sladek (2002).Repression of hepatocyte nuclear factor 4a by tumor suppressor p53: involvement of the ligand binding domain and histone deacetylase activity. Molecular Endocrinology, 16:402-410.

B. Wang, S-R. Cai, C. Gao, F.M. Sladek, K.P. Ponder (2001). Lipopolysaccharide results in a marked decrease in hepatocyte nuclear factor 4a in rat liver. Hepatology, 34:979-989.

Sladek, F.M., and S. Seidel (2001). "Hepatocyte Nuclear Factor 4a" book chapter in Nuclear Receptors and Genetic Disease (eds. T. P. Burris & E. McCabe, Academic Press, London).

Li, QJ., S. Vaingankar, F.M. Sladek, & M. Martins-Green (2000). Novel Nuclear Target for Thrombin: Activation of the Elk1 Transcription Factor Leads to Chemokine Gene Expression. Blood 96: 3696-3706.

Bogan* A., Q. Dallas-Yang*, M.D. Ruse*, Jr., Y. Maeda, G. Jiang, L. Nepomuceno, T. Scanlan, F. E. Cohen and F.M. Sladek (2000). Analysis of Protein Dimerization and Ligand Binding of Orphan receptor HNF4a. Journal of Molecular Biology, 302: 831-851. *Contributed equally

Sladek, F.M., M.D. Ruse, Jr., L. Nepomuceno, S.-M. Huang, M.R. Stallcup (1999) Modulation of Transcriptional Activation by a Splicing Variation in the F domain of Nuclear Receptor HNF4a, Molecular and Cellular Biology 19: 6509-6522.

Nuclear Receptor Nomenclature Committee (1999). A unified nomenclature system for the nuclear receptor superfamily, Cell 97: 161-163. (F.M. Sladek, endorsing author)

Sladek, F.M. Q. Dallas-Yang and L. Nepomuceno (1998). The MODY1 mutation in hepatocyte nuclear factor 4a still allows for dimerization in solution but causes abnormal sub cellular localization, Diabetes 47:985-990.

Dallas-Yang, G. Jiang and F.M. Sladek (1998). Avoidance of false positives in colony PCR, Biotechniques 24: 580-582.

Jiang, G., U. Lee and F.M. Sladek (1997). Proposed mechanism for the stabilization of nuclear receptor DNA binding via protein dimerization, Molecular and Cellular Biology 17:6546-6554.

Jiang, G.and F.M. Sladek (1997). The DNA binding domain of hepatocyte nuclear factor 4 (HNF-4) mediates cooperative, specific binding to DNA and heterodimerization with the retinoid X receptor a, Journal of Biological Chemistry 272: 1218-1225.

Jiang, G., L. Nepomuceno, Q. Yang and F.M. Sladek (1997). Serine/threonine phosphorylation of orphan receptor hepatocyte nuclear factor 4, Archives of Biochemistry and Biophysics 340:1-9.

Marten, N., F.M. Sladek, and D. S. Straus (1996). Effect of dietary protein restriction on liver transcription factors, Biochemical Journal 317: 361-370.

Hall, R.K., F.M. Sladek, and D. K. Granner (1995). The orphan receptors COUP-TF and HNF-4 serve as accessory factors required for induction of phosphoenolpyruvate carboxykinase gene transcription by glucocorticoids, Proc. Natl. Acad. Sci. USA 92: 412-416.

Jiang, G., L. Nepomuceno, K. Hopkins and F.M. Sladek (1995). Exclusive homodimerization of orphan receptor hepatocyte nuclear factor 4 defines a new subfamily of nuclear receptors, Molecular and Cellular Biology 15: 5131-5143.

Sladek, F.M. "Hepatocyte Nuclear factor 4 (HNF-4)" chapter in Liver Gene Expression, 1994 (ed.s F. Tronche, M. Yaniv, R.G. Landes Co., Austin, TX).

Zhong, W.,F.M. Sladek, and J. E. Darnell, Jr. (1993). The expression pattern of a Drosophila homologue to the mouse transcription factor HNF-4 suggests a determinative role in gut formation. EMBO Journal Vol. 12, No. 2: 537-544.

Metzger, S., J. L. Halaas, J. L. Breslow and F.M. Sladek (1993). Orphan receptor HNF-4 and bZip protein C/EBPa bind to overlapping regions of the apolipoprotein B gene promoter and synergistically activate transcription, Journal of Biological Chemistry 268: 16831-16838.

Sladek, F.M. (1993). Orphan receptor HNF-4 and liver-specific gene expression. Receptor 3: 223-232.

Reijnen, M. J., F.M. Sladek, R. M. Bertina and P. H. Reitsma (1992). Disruption of a binding site for hepatocyte nuclear factor 4 results in Hemophilia B. Leyden, Proc. Natl. Acad. Sci. USA 89: 6300-6303.

Mietus-Snyder, M.,F.M. Sladek, G. S. Ginsburg, C. F. Kuo, J.A.A. Ladias, J. E. Darnell, Jr. and S. K. Karathanasis (1992). Antagonism between apolipoportein AI regulatory protein 1, Ear3/Coup-TF and hepatocyte nuclear factor 4 modulates apolipoprotein CIII gene expression in liver and intestinal cells, Molecular and Cellular Biology 12: 1708-1718.

Sladek, F. M. and J. E. Darnell, Jr. (1992). Mechanisms of Liver-Specific Gene Expression, Current Opinions in Genetics and Development 2: 256-259.

Avraham, K. B., V. R. Prezioso, W. S. Chen, E. Lai,F.M. Sladek, W. Zhong, J. E. Darnell, Jr., N. A. Jenkins, and N. G. Copeland (1992) Murine Chromosomal Location of four Hepatocyte-Enriched Transcription Factors: HNF-3a, HNF-3b, HNF-3g, and HNF-4, Genomics 13: 264-268.

Kuo, C. J., P. B. Conley, L. Chen, F.M. Sladek, J. E. Darnell, Jr., and G. R. Crabtree (1992). A transcriptional hierarchy involved in mammalian cell-type specification, Nature 355: 457-461.

Herbst, R.S., U. Nielsch, F.M. Sladek, E. Lai, L.E. Babiss and J.E. Darnell, Jr. (1991). Decreased transcription factor concentration explains the limited hepatocyte-like transcription in hepatoma cells, New Biologist 3: 289-296.

Xanthopoulos, K.G., V.R. Prezioso, W.S. Chen, F.M. Sladek, R. Cortese and J.E. Darnell, Jr., (1991) The different tissue transcription patterns of genes for HNF-1, C/EBP, HNF-3 and HNF-4, protein factors that govern liver-specific transcription, Proc. Natl. Acad. Sci. USA 88:3807-3811.

Sladek, F.M., W. Zhong, E. Lai and J.E. Darnell, Jr. (1990). Liver-enriched transcription factor HNF-4 is a novel member of the steroid hormone receptor superfamily, Genes and Development 4: 2353-2365.

Sladek, F.M., A. Melian and P. Howard-Flanders (1989). Incision by UvrABC excinuclease is a step in the path to mutagenesis by psoralen crosslinks in Escherichia coli, Proc. Natl. Acad. Sci. USA 86, 3982-3986.

Sladek, F.M., M.M. Munn, W.D. Rupp and P. Howard-Flanders (1989) In Vitro Repair of Psoralen-DNA Crosslinks by RecA, UvrABC and the 5'-exonuclease of DNA Polymerase I, Journal of Biological Chemistry 264, 6755-6765.